Gill:10 X M9 salts + glucose

From OpenWetWare

(Difference between revisions)
Jump to: navigation, search
(New page: ==Materials== 1000 mL: *dH<sub>2</sub>O 700 ml *Na<sub>2</sub>HPO<sub>4</sub> • 7H<sub>2</sub>O 128 g *KH<sub>2</sub>PO<sub>4</sub> 30 g *NaCl 5 g *NH<sub>4</sub>Cl 10 g *Glucose 40 g ...)
Current revision (17:11, 23 April 2013) (view source)
m
 
Line 3: Line 3:
1000 mL:
1000 mL:
-
*dH<sub>2</sub>O 700 ml
+
*700 ml dH<sub>2</sub>O
-
*Na<sub>2</sub>HPO<sub>4</sub> • 7H<sub>2</sub>O 128 g
+
*128 g Na<sub>2</sub>HPO<sub>4</sub> • 7H<sub>2</sub>O
-
*KH<sub>2</sub>PO<sub>4</sub> 30 g
+
*30 g KH<sub>2</sub>PO<sub>4</sub>
-
*NaCl 5 g
+
*5 g NaCl
-
*NH<sub>4</sub>Cl 10 g
+
*10 g NH<sub>4</sub>Cl
-
*Glucose 40 g
+
*40 g Glucose
==Procedure==
==Procedure==

Current revision

Materials

1000 mL:

  • 700 ml dH2O
  • 128 g Na2HPO4 • 7H2O
  • 30 g KH2PO4
  • 5 g NaCl
  • 10 g NH4Cl
  • 40 g Glucose

Procedure

Add all components to water, dissolve, adjust to 1000 ml. Filter sterilize at 0.22 µm (do not autoclave), store at RT. If using anhydrous Na2HPO4, multiply the weight for this component by 0.53.

Notes

Please feel free to post comments, questions, or improvements to this protocol. Happy to have your input!

Personal tools