Gill:10x Lambda diluent (no gelatin)
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Materials
1000 mL:
- Tris base 60.56 g
- NaCl 58.0 g
- MgSO4 10 g
- dH2O to 1000 ml
Procedure
Dissolve in 800 ml dH2O, adjust pH to 7.5 with HCl, add water to a final volume of 1000 ml. Autoclave, store at room temp.
Notes
Please feel free to post comments, questions, or improvements to this protocol. Happy to have your input!