Gill:50X Vogel-Bonner salts: Difference between revisions
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(New page: ==Materials== {| class="wikitable" |- ! ! 1000 mL |- |Warm dd H2O (45°C) |670 ml |- |MgSO4 • 7 H2O |10 g |- |Citric acid monohydrate |100 g |- |K2HPO4 |500 g |- ...) |
(No difference)
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Revision as of 13:41, 23 April 2013
Materials
| 1000 mL | |
|---|---|
| Warm dd H2O (45°C) | 670 ml |
| MgSO4 • 7 H2O | 10 g |
| Citric acid monohydrate | 100 g |
| K2HPO4 | 500 g |
| NaHNH4PO4 • 4H2O | 175 g |
Procedure
Add salts in the order indicated to warm water in a 2-liter flask placed on magnetic stirring hot plate. Allow each salt to dissolve completely before adding the next. Adjust the volume to 1 liter. Distribute into two 1-liter glass bottles. Autoclave for 20 min at 121°C, store at RT.
Notes
Please feel free to post comments, questions, or improvements to this protocol. Happy to have your input!
