Gill:Lambda diluent (SM buffer)

From OpenWetWare

(Difference between revisions)
Jump to: navigation, search
(New page: ==Materials== 1000 mL: * 1M Tris-HCl, pH 7.5, 50 ml (25 mM) * NaCl 5.8 g (100 mM) * MgSO4 1 g (8 mM) * Gelatin (2% sol’n) 5 ml (0.01 %) * dH<sub>2</sub>O 475 ml ==Procedure== Mix all ...)
Line 3: Line 3:
1000 mL:
1000 mL:
-
* 1M Tris-HCl, pH 7.5, 50 ml (25 mM)
+
* 50 ml (25 mM) 1M Tris-HCl, pH 7.5
-
* NaCl 5.8 g (100 mM)
+
* 5.8 g (100 mM) NaCl
-
* MgSO4 1 g (8 mM)
+
* 1 g (8 mM) MgSO4
-
* Gelatin (2% sol’n) 5 ml (0.01 %)
+
* 5 ml (0.01 %) Gelatin (2% sol’n)
-
* dH<sub>2</sub>O 475 ml
+
* 475 ml dH<sub>2</sub>O
==Procedure==
==Procedure==

Revision as of 16:59, 23 April 2013

Materials

1000 mL:

  • 50 ml (25 mM) 1M Tris-HCl, pH 7.5
  • 5.8 g (100 mM) NaCl
  • 1 g (8 mM) MgSO4
  • 5 ml (0.01 %) Gelatin (2% sol’n)
  • 475 ml dH2O

Procedure

Mix all ingredients, then aliquot 100 ml into square milk bottles & autoclave. Let bottles cool and tighten caps, store at room temp.

Notes

Please feel free to post comments, questions, or improvements to this protocol. Happy to have your input!

Personal tools