Gill:Lambda diluent (SM buffer): Difference between revisions
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(New page: ==Materials== 1000 mL: * 1M Tris-HCl, pH 7.5, 50 ml (25 mM) * NaCl 5.8 g (100 mM) * MgSO4 1 g (8 mM) * Gelatin (2% sol’n) 5 ml (0.01 %) * dH<sub>2</sub>O 475 ml ==Procedure== Mix all ...) |
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| Line 3: | Line 3: | ||
1000 mL: | 1000 mL: | ||
* 1M Tris-HCl, pH 7.5 | * 50 ml (25 mM) 1M Tris-HCl, pH 7.5 | ||
* | * 5.8 g (100 mM) NaCl | ||
* | * 1 g (8 mM) MgSO4 | ||
* | * 5 ml (0.01 %) Gelatin (2% sol’n) | ||
* dH<sub>2</sub>O | * 475 ml dH<sub>2</sub>O | ||
==Procedure== | ==Procedure== | ||
Revision as of 13:59, 23 April 2013
Materials
1000 mL:
- 50 ml (25 mM) 1M Tris-HCl, pH 7.5
- 5.8 g (100 mM) NaCl
- 1 g (8 mM) MgSO4
- 5 ml (0.01 %) Gelatin (2% sol’n)
- 475 ml dH2O
Procedure
Mix all ingredients, then aliquot 100 ml into square milk bottles & autoclave. Let bottles cool and tighten caps, store at room temp.
Notes
Please feel free to post comments, questions, or improvements to this protocol. Happy to have your input!
