Gill:Lambda diluent (SM buffer)

From OpenWetWare

Revision as of 16:59, 23 April 2013 by Jacqueline K. Grimm (Talk | contribs)
Jump to: navigation, search

Materials

1000 mL:

  • 50 ml (25 mM) 1M Tris-HCl, pH 7.5
  • 5.8 g (100 mM) NaCl
  • 1 g (8 mM) MgSO4
  • 5 ml (0.01 %) Gelatin (2% sol’n)
  • 475 ml dH2O

Procedure

Mix all ingredients, then aliquot 100 ml into square milk bottles & autoclave. Let bottles cool and tighten caps, store at room temp.

Notes

Please feel free to post comments, questions, or improvements to this protocol. Happy to have your input!

Personal tools