Gill:M9 agar

Materials

500 mL:

• Bacto agar 7.5 g
• dH₂O 450 ml
• 10 X M9 salts + glucose 50 ml
• 1 M MgSO₄ 1 ml (2 mM final)
• 1 M CaCl₂ 50 µl (0.1 mM final)

1000 mL:

• Bacto agar 15 g
• dH₂O 880 ml
• 10 X M9 salts + glucose 100 ml
• 1 M MgSO₄ 2 ml (2 mM final)
• 1 M CaCl₂ 0.1 ml (0.1 mM final)

Procedure

Autoclave agar/water solution, let cool. Aseptically add sterile salts + glucose, pour.

Notes

Please feel free to post comments, questions, or improvements to this protocol. Happy to have your input!