Gill:Protocols: Difference between revisions

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[[Gill:S. aureus genomic DNA isolation |S. aureus genomic DNA isolation]]
[[Gill:S. aureus genomic DNA isolation |S. aureus genomic DNA isolation]]
[[Gill:Subculturing phage isolates |Subculturing phage isolates]]


===Solutions and Media Preparation===
===Solutions and Media Preparation===

Revision as of 15:55, 11 November 2013

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Protocols

Microbiology and Molecular Biology

Chung cells

Confirming phage via gel electrophoresis

Making frozen permanents

Making plate lysates

Phage genomic DNA extraction

S. aureus genomic DNA isolation

Subculturing phage isolates

Solutions and Media Preparation

Lambda diluent (SM buffer)

10x Lambda diluent (no gelatin)

LB agar

LB broth

M9 agar

Supplemented M9 agar (M9sup)

Supplemented M9 broth (M9sup)

10 X M9 salts

10 X M9 salts + glucose

S liquid medium

TNA (Tryptone Nutrient Agar)

TNA-SA

TNB (Tryptone Nutrient Broth)

Tris-HCl

TSA (Tryptone Soy Agar)

TSB (Tryptone Soy Broth)

T-top (0.5% agar)

Vogel-Bonner glucose (VBG) minimal medium

50X Vogel-Bonner salts

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