Gill:Protocols: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
No edit summary |
No edit summary |
||
| (24 intermediate revisions by 2 users not shown) | |||
| Line 3: | Line 3: | ||
==Protocols== | ==Protocols== | ||
=== | ===Microbiology and Molecular Biology=== | ||
[[Gill:Calcium chloride competent cells |Calcium chloride competent cells]] | |||
[[Gill:Chung cells |Chung cells]] | |||
[[Gill:Confirming phage via gel electrophoresis |Confirming phage via restriction digest and gel electrophoresis]] | |||
[[Gill:Making frozen permanents |Making frozen permanents]] | |||
[[Gill:Making plate lysates |Making plate lysates]] | |||
[[Gill:Phage enrichment |Phage enrichment]] | |||
[[Gill:Phage genomic DNA extraction |Phage genomic DNA extraction]] | |||
[[Gill:Plating out phage |Plating out phage]] | |||
[[Gill:Preparing phage specimens for TEM |Preparing phage specimens for TEM]] | |||
[[Gill:Rapid bacterial DNA prep |Rapid bacterial DNA prep]] | |||
[[Gill:S. aureus genomic DNA isolation |S. aureus genomic DNA isolation]] | |||
[[Gill: | [[Gill:S. aureus pre-treatment for plasmid preps |S. aureus pre-treatment for plasmid preps]] | ||
[[Gill: | [[Gill:Subculturing phage isolates |Subculturing phage isolates]] | ||
===Solutions and Media Preparation=== | |||
[[Gill: | [[Gill:Lambda diluent (SM buffer) |Lambda diluent (SM buffer)]] | ||
[[Gill:Lambda diluent ( | [[Gill:10x Lambda diluent (no gelatin) |10x Lambda diluent (no gelatin)]] | ||
[[Gill: | [[Gill:LB agar |LB agar]] | ||
[[Gill: | [[Gill:LB broth |LB broth]] | ||
[[Gill: | [[Gill:M9 agar |M9 agar]] | ||
[[Gill: | [[Gill:Supplemented M9 agar (M9sup) |Supplemented M9 agar (M9sup)]] | ||
[[Gill: | [[Gill:Supplemented M9 broth (M9sup) |Supplemented M9 broth (M9sup)]] | ||
[[Gill: | [[Gill:10 X M9 salts |10 X M9 salts]] | ||
[[Gill: | [[Gill:10 X M9 salts + glucose |10 X M9 salts + glucose]] | ||
[[Gill: | [[Gill:S liquid medium |S liquid medium]] | ||
[[Gill: | [[Gill:TNA (Tryptone Nutrient Agar) |TNA (Tryptone Nutrient Agar)]] | ||
[[Gill: | [[Gill:TNA-SA |TNA-SA]] | ||
[[Gill: | [[Gill:TNB (Tryptone Nutrient Broth) |TNB (Tryptone Nutrient Broth)]] | ||
[[Gill: | [[Gill:Tris-HCl |Tris-HCl]] | ||
[[Gill: | [[Gill:TSA (Tryptone Soy Agar) |TSA (Tryptone Soy Agar)]] | ||
[[Gill: | [[Gill:TSB (Tryptone Soy Broth) |TSB (Tryptone Soy Broth)]] | ||
[[Gill: | [[Gill:T-top (0.5% agar) |T-top (0.5% agar)]] | ||
[[Gill: | [[Gill:Vogel-Bonner glucose (VBG) minimal medium |Vogel-Bonner glucose (VBG) minimal medium]] | ||
[[Gill: | [[Gill:50X Vogel-Bonner salts |50X Vogel-Bonner salts]] | ||
Revision as of 13:19, 27 May 2014
Protocols
Microbiology and Molecular Biology
Calcium chloride competent cells
Confirming phage via restriction digest and gel electrophoresis
Preparing phage specimens for TEM
S. aureus genomic DNA isolation
S. aureus pre-treatment for plasmid preps
Solutions and Media Preparation
10x Lambda diluent (no gelatin)
