Gill:Protocols

From OpenWetWare

(Difference between revisions)
Jump to: navigation, search
Line 10: Line 10:
===Solutions and Media Preparation===
===Solutions and Media Preparation===
-
 
-
[[Gill:TNA (Tryptone Nutrient Agar) |TNA (Tryptone Nutrient Agar)]]
 
-
 
-
[[Gill:TNB (Tryptone Nutrient Broth) |TNB (Tryptone Nutrient Broth)]]
 
-
 
-
[[Gill:TNA-SA |TNA-SA]]
 
-
 
-
[[Gill:Tris-HCl |Tris-HCl]]
 
[[Gill:Lambda diluent (SM buffer) |Lambda diluent (SM buffer)]]
[[Gill:Lambda diluent (SM buffer) |Lambda diluent (SM buffer)]]
Line 23: Line 15:
[[Gill:10x Lambda diluent (no gelatin) |10x Lambda diluent (no gelatin)]]
[[Gill:10x Lambda diluent (no gelatin) |10x Lambda diluent (no gelatin)]]
-
[[Gill:TSB (Tryptone Soy Broth) |TSB (Tryptone Soy Broth)]]
+
[[Gill:LB agar |LB agar]]
-
 
+
-
[[Gill:TSA (Tryptone Soy Agar) |TSA (Tryptone Soy Agar)]]
+
[[Gill:LB broth |LB broth]]
[[Gill:LB broth |LB broth]]
-
[[Gill:LB agar |LB agar]]
+
[[Gill:M9 agar |M9 agar]]
-
[[Gill:S liquid medium |S liquid medium]]
+
[[Gill:Supplemented M9 agar (M9sup) |Supplemented M9 agar (M9sup)]]
-
[[Gill:T-top (0.5% agar) |T-top (0.5% agar)]]
+
[[Gill:Supplemented M9 broth (M9sup) |Supplemented M9 broth (M9sup)]]
[[Gill:10 X M9 salts |10 X M9 salts]]
[[Gill:10 X M9 salts |10 X M9 salts]]
Line 39: Line 29:
[[Gill:10 X M9 salts + glucose |10 X M9 salts + glucose]]
[[Gill:10 X M9 salts + glucose |10 X M9 salts + glucose]]
-
[[Gill:M9 agar |M9 agar]]
+
[[Gill:S liquid medium |S liquid medium]]
-
[[Gill:Supplemented M9 agar (M9sup) |Supplemented M9 agar (M9sup)]]
+
[[Gill:TNA (Tryptone Nutrient Agar) |TNA (Tryptone Nutrient Agar)]]
-
[[Gill:Supplemented M9 broth (M9sup) |Supplemented M9 broth (M9sup)]]
+
[[Gill:TNA-SA |TNA-SA]]
 +
 
 +
[[Gill:TNB (Tryptone Nutrient Broth) |TNB (Tryptone Nutrient Broth)]]
 +
 
 +
[[Gill:Tris-HCl |Tris-HCl]]
 +
 
 +
[[Gill:TSA (Tryptone Soy Agar) |TSA (Tryptone Soy Agar)]]
 +
 
 +
[[Gill:TSB (Tryptone Soy Broth) |TSB (Tryptone Soy Broth)]]
 +
 
 +
[[Gill:T-top (0.5% agar) |T-top (0.5% agar)]]
[[Gill:Vogel-Bonner glucose (VBG) minimal medium |Vogel-Bonner glucose (VBG) minimal medium]]
[[Gill:Vogel-Bonner glucose (VBG) minimal medium |Vogel-Bonner glucose (VBG) minimal medium]]
[[Gill:50X Vogel-Bonner salts |50X Vogel-Bonner salts]]
[[Gill:50X Vogel-Bonner salts |50X Vogel-Bonner salts]]

Revision as of 17:20, 7 November 2013

Home        Research        Protocols        Lab Members        Publications        Internal        Contact       


Protocols

Microbiology

Chung cells
Confirming phage via gel electrophoresis
Making frozen permanents
S. aureus genomic DNA isolation

Solutions and Media Preparation

Lambda diluent (SM buffer)

10x Lambda diluent (no gelatin)

LB agar

LB broth

M9 agar

Supplemented M9 agar (M9sup)

Supplemented M9 broth (M9sup)

10 X M9 salts

10 X M9 salts + glucose

S liquid medium

TNA (Tryptone Nutrient Agar)

TNA-SA

TNB (Tryptone Nutrient Broth)

Tris-HCl

TSA (Tryptone Soy Agar)

TSB (Tryptone Soy Broth)

T-top (0.5% agar)

Vogel-Bonner glucose (VBG) minimal medium

50X Vogel-Bonner salts

Personal tools