Gill:S liquid medium: Difference between revisions
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| Line 6: | Line 6: | ||
1000 mL: | 1000 mL: | ||
*Yeast extract | *1 g Yeast extract | ||
*Na<sub>2</sub>HPO<sub>4</sub> | *10 g Na<sub>2</sub>HPO<sub>4</sub> | ||
*KH<sub>2</sub>PO<sub>4</sub> | *3 g KH<sub>2</sub>PO<sub>4</sub> | ||
*K<sub>2</sub>SO<sub>4</sub> | *1 g K<sub>2</sub>SO<sub>4</sub> | ||
* | *1 g NaCl | ||
* | *900 mL Water | ||
==Procedure== | ==Procedure== | ||
Latest revision as of 14:08, 23 April 2013
Overview
For Bcc EPS production.
Materials
1000 mL:
- 1 g Yeast extract
- 10 g Na2HPO4
- 3 g KH2PO4
- 1 g K2SO4
- 1 g NaCl
- 900 mL Water
Procedure
Dissolve above components in 900 ml water and autoclave. Before use, add the following components from sterile stock solutions:
| 20 % Glucose | 100 ml | (2% final) |
| 10 % Casamino acids | 10 ml | (0.1% final) |
| MgSO4 (from 1 M stock) | 1 ml | (1 mM final) |
| CaCl2 (from 1 M stock) | 0.1 ml | (0.1 mM final) |
| FeSO4 (from 0.1 M stock) | 50 µl | (5 µM final) |
Notes
Please feel free to post comments, questions, or improvements to this protocol. Happy to have your input!
