Gill:Supplemented M9 agar (M9sup)

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(New page: ==Materials== {| class="wikitable" |- ! ! 500 mL ! 1000 mL |- | Bacto Agar | 7.5 g | 15 g |- | dH<sub>2</sub>O | 430 ml | 860 ml |- | 10 X M9 salts + glucose | 50 ml | 100 ml |- | 10% ...)
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Materials

500 mL 1000 mL
Bacto Agar 7.5 g 15 g
dH2O 430 ml 860 ml
10 X M9 salts + glucose 50 ml 100 ml
10% Casamino acids 10 ml 20 ml (0.2 % final)
1% Thiamine 0.5 ml 1 ml (10 µg/ml final)
1 M MgSO4 1 ml 2 ml (2 mM final)
1 M CaCl2 50 µl 0.1 ml (0.1 mM final)

Procedure

Autoclave agar/water solution, let cool. Aseptically add sterile salts, glucose, casamino acids, and thiamine, pour.

Notes

Please feel free to post comments, questions, or improvements to this protocol. Happy to have your input!

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