# Gill:Supplemented M9 broth (M9sup)

### From OpenWetWare

## Materials

50 mL | 500 mL | 1000 mL | |
---|---|---|---|

dH_{2}O
| 43 ml | 430 ml | 860 ml |

10 X M9 salts + glucose | 5 ml | 50 ml | 100 ml |

10% Casamino acids | 1 ml | 10 ml | 20 ml |

1% Thiamine | 50 µl | 0.5 ml | 1 ml |

1 M MgSO_{4}
| 100 µl | 1 ml | 2 ml |

1 M CaCl_{2}
| 5 µl | 50 µl | 0.1 ml |

## Procedure

Pre-sterilize all component solutions and aseptically add to sterile water. Alternatively, mix all components and then filter-sterilize through a 0.22 µm filter. Store at 4 ºC.

## Notes

Please feel free to post comments, questions, or improvements to this protocol. Happy to have your input!