Gill:T-top (0.5% agar): Difference between revisions
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(New page: ==Materials== 500 mL: *Tryptone 5 g *NaCl 5 g *dH<sub>2</sub>O 500 ml *Bacto agar 1000 mL: *Tryptone 10 g *NaCl 10 g *dH<sub>2</sub>O 1000 ml *Bacto agar ==Procedure== D...) |
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| Line 3: | Line 3: | ||
500 mL: | 500 mL: | ||
* | *5 g Tryptone | ||
* | *5 g NaCl | ||
*dH<sub>2</sub>O | *500 ml dH<sub>2</sub>O | ||
*Bacto agar | *Bacto agar | ||
1000 mL: | 1000 mL: | ||
* | *10 g Tryptone | ||
* | *10 g NaCl | ||
*dH<sub>2</sub>O | *1000 ml dH<sub>2</sub>O | ||
*Bacto agar | *Bacto agar | ||
Latest revision as of 14:09, 23 April 2013
Materials
500 mL:
- 5 g Tryptone
- 5 g NaCl
- 500 ml dH2O
- Bacto agar
1000 mL:
- 10 g Tryptone
- 10 g NaCl
- 1000 ml dH2O
- Bacto agar
Procedure
Dissolve tryptone and NaCl in water. Add Bacto Agar to square milk bottles to a final concentration of 0.5 % w/v (0.25 g / 50 ml, 0.5 g / 100 ml). Aliquot medium to bottles as appropriate (50 or 100 ml), autoclave and store at RT. To use, melt an aliquot of top agar in a microwave and add MgSO4 and CaCl2 from sterile 1 M stocks to a final concentration of 5 mM each.
Notes
Please feel free to post comments, questions, or improvements to this protocol. Happy to have your input!
