Gill:T-top (0.5% agar)

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(New page: ==Materials== 500 mL: *Tryptone 5 g *NaCl 5 g *dH<sub>2</sub>O 500 ml *Bacto agar 1000 mL: *Tryptone 10 g *NaCl 10 g *dH<sub>2</sub>O 1000 ml *Bacto agar ==Procedure== D...)
Current revision (16:09, 23 April 2013) (view source)
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500 mL:
500 mL:
-
*Tryptone 5 g
+
*5 g Tryptone
-
*NaCl 5 g
+
*5 g NaCl
-
*dH<sub>2</sub>O 500 ml
+
*500 ml dH<sub>2</sub>O
*Bacto agar
*Bacto agar
1000 mL:
1000 mL:
-
*Tryptone 10 g
+
*10 g Tryptone
-
*NaCl 10 g
+
*10 g NaCl
-
*dH<sub>2</sub>O 1000 ml
+
*1000 ml dH<sub>2</sub>O
*Bacto agar
*Bacto agar

Current revision

Materials

500 mL:

  • 5 g Tryptone
  • 5 g NaCl
  • 500 ml dH2O
  • Bacto agar

1000 mL:

  • 10 g Tryptone
  • 10 g NaCl
  • 1000 ml dH2O
  • Bacto agar

Procedure

Dissolve tryptone and NaCl in water. Add Bacto Agar to square milk bottles to a final concentration of 0.5 % w/v (0.25 g / 50 ml, 0.5 g / 100 ml). Aliquot medium to bottles as appropriate (50 or 100 ml), autoclave and store at RT. To use, melt an aliquot of top agar in a microwave and add MgSO4 and CaCl2 from sterile 1 M stocks to a final concentration of 5 mM each.

Notes

Please feel free to post comments, questions, or improvements to this protocol. Happy to have your input!

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