Gill:T-top (0.5% agar)

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Revision as of 17:09, 23 April 2013 by Jacqueline K. Grimm (Talk | contribs)
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Materials

500 mL:

  • 5 g Tryptone
  • 5 g NaCl
  • 500 ml dH2O
  • Bacto agar

1000 mL:

  • 10 g Tryptone
  • 10 g NaCl
  • 1000 ml dH2O
  • Bacto agar

Procedure

Dissolve tryptone and NaCl in water. Add Bacto Agar to square milk bottles to a final concentration of 0.5 % w/v (0.25 g / 50 ml, 0.5 g / 100 ml). Aliquot medium to bottles as appropriate (50 or 100 ml), autoclave and store at RT. To use, melt an aliquot of top agar in a microwave and add MgSO4 and CaCl2 from sterile 1 M stocks to a final concentration of 5 mM each.

Notes

Please feel free to post comments, questions, or improvements to this protocol. Happy to have your input!

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