Gill:TNA (Tryptone Nutrient Agar): Difference between revisions
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(New page: ==Materials== 500 mL: *Tryptone 2.5 g *Yeast extract 1.25 g *Glucose 0.5 g *NaCl 4.25 g *Agar 7.5 g *dH<sub>2</sub>O 500 ml ==Procedure== Mix all ingredients in a 1 litre bott...) |
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Revision as of 12:42, 23 April 2013
Materials
500 mL:
- Tryptone 2.5 g
- Yeast extract 1.25 g
- Glucose 0.5 g
- NaCl 4.25 g
- Agar 7.5 g
- dH2O 500 ml
Procedure
Mix all ingredients in a 1 litre bottle or flask, cover and autoclave. Label caps with “TNA” to avoid confusion. Immediately after autoclaving, swirl media to dissolve the melted agar. Tighten caps and place bottles in the water bath. Pour plates when the agar temperature is 50-60 ºC.
Notes
Please feel free to post comments, questions, or improvements to this protocol. Happy to have your input!
