Gill:TNA (Tryptone Nutrient Agar): Difference between revisions
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(New page: ==Materials== 500 mL: *Tryptone 2.5 g *Yeast extract 1.25 g *Glucose 0.5 g *NaCl 4.25 g *Agar 7.5 g *dH<sub>2</sub>O 500 ml ==Procedure== Mix all ingredients in a 1 litre bott...) |
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| Line 3: | Line 3: | ||
500 mL: | 500 mL: | ||
* | *2.5 g Tryptone | ||
* | *1.25 g Yeast extract | ||
* | *0.5 g Glucose | ||
* | *4.25 g NaCl | ||
* | *7.5 g Agar | ||
*dH<sub>2</sub>O | *500 ml dH<sub>2</sub>O | ||
==Procedure== | ==Procedure== | ||
Latest revision as of 13:55, 23 April 2013
Materials
500 mL:
- 2.5 g Tryptone
- 1.25 g Yeast extract
- 0.5 g Glucose
- 4.25 g NaCl
- 7.5 g Agar
- 500 ml dH2O
Procedure
Mix all ingredients in a 1 litre bottle or flask, cover and autoclave. Label caps with “TNA” to avoid confusion. Immediately after autoclaving, swirl media to dissolve the melted agar. Tighten caps and place bottles in the water bath. Pour plates when the agar temperature is 50-60 ºC.
Notes
Please feel free to post comments, questions, or improvements to this protocol. Happy to have your input!
