From OpenWetWare

Revision as of 15:51, 23 April 2013 by Jacqueline K. Grimm (Talk | contribs)
(diff) ←Older revision | Current revision (diff) | Newer revision→ (diff)
Jump to: navigation, search

1M Tris-HCl, pH 7.5


1000 mL:

  • Tris base 121.12 g
  • dH2O 800 mL
  • HCl


Dissolve in 800 ml dH2O, adjust pH to 7.5 with HCl, add water to a final volume of 1000 ml. Aliquot as appropriate, autoclave, store at room temp.


Please feel free to post comments, questions, or improvements to this protocol. Happy to have your input!

Personal tools