From OpenWetWare

Revision as of 16:57, 23 April 2013 by Jacqueline K. Grimm (Talk | contribs)
Jump to: navigation, search

1M Tris-HCl, pH 7.5


1000 mL:

  • 121.12 g Tris base
  • 800 mL dH2O
  • HCl


Dissolve in 800 ml dH2O, adjust pH to 7.5 with HCl, add water to a final volume of 1000 ml. Aliquot as appropriate, autoclave, store at room temp.


Please feel free to post comments, questions, or improvements to this protocol. Happy to have your input!

Personal tools