Godin:Protocols/Lambda DNA Aliquots: Difference between revisions
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==Lambda DNA Aliquots== | ==Lambda DNA Aliquots== | ||
In order to avoid contamination of the stock solution of Lambda DNA, aliquots must be | In order to avoid contamination of the stock solution of Lambda DNA, aliquots must be made. | ||
An aliquot is created by combining the following solutions in a microcentrifuge tube: | |||
*10μL of stock 500μg/mL Lambda-DNA | *10μL of stock 500μg/mL Lambda-DNA | ||
*10μL of solution containing 2 mol/L KCl, 20 mmol/L HEPES, 2 mmol/L EDTA brought to a pH of 7 | *10μL of solution containing 2 mol/L KCl, 20 mmol/L HEPES, 2 mmol/L EDTA brought to a pH of 7 | ||
Revision as of 07:00, 7 June 2010
Lambda DNA Aliquots
In order to avoid contamination of the stock solution of Lambda DNA, aliquots must be made.
An aliquot is created by combining the following solutions in a microcentrifuge tube:
- 10μL of stock 500μg/mL Lambda-DNA
- 10μL of solution containing 2 mol/L KCl, 20 mmol/L HEPES, 2 mmol/L EDTA brought to a pH of 7
- 80μL of solution containing 1 mol/L KCl, 10 mmol/L HEPES, 1 mmol/L EDTA brought to a pH of 7
Once combined, gently mix the aliquot and store at -20°C
To use for tesing, inject 10μL of the aliquots into the 500μL Teflon cell volume. This will result in a 1μg/mL DNA concentration for recording translocation events.
