Godin:Protocols/Microscopes: Difference between revisions
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Chloe Promsy (talk | contribs) (New page: ==Abstract== Measure thickness and lateral size of sample with the inverted microscope ===Equipment=== inverted microscope : Olympus BX41 Microscope camera : Cool snap HQ2 ==Procedure== ...) |
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Revision as of 08:28, 10 June 2010
Abstract
Measure thickness and lateral size of sample with the inverted microscope
Equipment
inverted microscope : Olympus BX41 Microscope camera : Cool snap HQ2
Procedure
For thickness (Z):
- Focus on top of your sample. - Read the graduation on the fine adjustment knob. - Focus on the bottom of your sample. - Read the graduation on the fine adjustment knob. - Subtract two readings. - Multiply this number by 1.2 to have your thickness in micrometer approximately.
For lateral size (X,Y):
- Take a picture of your sample. - For 10x50x → 1pixel≈129.7μm - For 10x20x → 1pixel≈325.7μm