Godin:Protocols/Multilayer PDMS

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*[http://en.wikipedia.org/wiki/Isopropanol Isopropanol]
*[http://en.wikipedia.org/wiki/Isopropanol Isopropanol]
*Stereo microscope
*Stereo microscope
 +
*''Scotch'' tape
*Pipette tip
*Pipette tip
*Tweezers
*Tweezers
*Polystyrene dish
*Polystyrene dish
 +
*Vacuum
*Oven
*Oven
*Aluminum foil
*Aluminum foil
*Weighing dish
*Weighing dish
*Scale
*Scale
-
*PDMS
+
*[http://www.dowcorning.com/applications/search/products/Details.aspx?prod=01064291&type=PROD PDMS]
*[[Godin:Protocols/Sonicator]]
*[[Godin:Protocols/Sonicator]]
*[[Godin:Protocols/Spin coater]]
*[[Godin:Protocols/Spin coater]]
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*'''Mix PDMS 7:1'''
*'''Mix PDMS 7:1'''
-
 
+
For most devices, 80g of PDMS is sufficient to produce reasonably-sized devices. Weigh 70g of silicone elastomer base on the scale using the weighing dish, then add 10g of curing agent. Make sure to maintain the 7:1 ratio; if too much is added, add more base as required. Use a pipette tip to mix the solution for 10 min under the fume hood. Blow dry the valve master with nitrogen, and place in a polystyrene dish. Use ''Scotch'' tape to fix the wafer at the center of the dish by taping all sides. We want to prevent the wafer from floating up, or PDMS from getting under the wafer. Close the dish with the lid.
*'''30 min degass'''
*'''30 min degass'''
 +
 +
Place
 +
*'''12 min @ 80°C'''
*'''12 min @ 80°C'''
*'''Punch valve holes'''
*'''Punch valve holes'''

Revision as of 11:36, 17 May 2011

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Contents

Safety

  • Safety goggles
  • Nitrile gloves
  • Lab coat
  • Clean room gowning
  • Fume hood

Materials

Description

This protocol details the fabrication of a 2 layer PDMS device, consisting of a valve layer (top) and channel layer (below). It is assumed that masters for respective layers have already been fabricated (see Godin:Protocols/SU-8 Master, Godin:Protocols/AZ Master). The idea is to modify the silicone elastomer base to curing agent ratio, thereby modifying PDMS elasticity.

Valve Layer

  • Mix PDMS 7:1

For most devices, 80g of PDMS is sufficient to produce reasonably-sized devices. Weigh 70g of silicone elastomer base on the scale using the weighing dish, then add 10g of curing agent. Make sure to maintain the 7:1 ratio; if too much is added, add more base as required. Use a pipette tip to mix the solution for 10 min under the fume hood. Blow dry the valve master with nitrogen, and place in a polystyrene dish. Use Scotch tape to fix the wafer at the center of the dish by taping all sides. We want to prevent the wafer from floating up, or PDMS from getting under the wafer. Close the dish with the lid.

  • 30 min degass

Place

  • 12 min @ 80°C
  • Punch valve holes
  • Cleaning

Fluidic Layer

  • Mix PDMS 20:1
  • 30 min degass
  • Spin coat
  • 10 min @ 80°C

Device Fabrication

  • Alignment
  • >2 hrs @ 80°C
  • Punch fluidic holes
  • Cleaning
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