Godin:Protocols/Piranha: Difference between revisions
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*Lab coat | *Lab coat | ||
*Lab apron | *Lab apron | ||
*Fume hood | *Fume hood <br> <br> | ||
==Materials== | ==Materials== | ||
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*Reaction vial and transport container | *Reaction vial and transport container | ||
*''Bluesorb'' wipes | *''Bluesorb'' wipes | ||
*2 pipettes and a rubber bulb | *2 pipettes and a rubber bulb <br> <br> | ||
==Description== | ==Description== | ||
Piranha is a powerful mix of sulfuric acid and hydrogen peroxide, used to clean microfluidic channels and render surfaces highly hydrophilic. Since it acts as an oxidizer, it is particularly useful against organics. The idea is to mix 1 part hydrogen peroxide into 3 parts sulphuric acid. For microfluidic cleansing, you'll only require a small amount (about 4ml). Don't make any more then you need! | Piranha is a powerful mix of sulfuric acid and hydrogen peroxide, used to clean microfluidic channels and render surfaces highly hydrophilic. Since it acts as an oxidizer, it is particularly useful against organics. The idea is to mix 1 part hydrogen peroxide into 3 parts sulphuric acid. For microfluidic cleansing, you'll only require a small amount (about 4ml). Don't make any more then you need! <br> <br> | ||
==Preparation== | ==Preparation== | ||
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#Rinse the pipette in the distilled water, then do so with water from fume hood faucet and discard in ''Broken Glass'' box. | #Rinse the pipette in the distilled water, then do so with water from fume hood faucet and discard in ''Broken Glass'' box. | ||
#Clean up any drops and replace hydrogen peroxide in the refrigerator. | #Clean up any drops and replace hydrogen peroxide in the refrigerator. | ||
#At this point you should observe bubbles making their way to the surface. Seal the transport container and bring it over over to your setup, you're all set! | #At this point you should observe bubbles making their way to the surface. Seal the transport container and bring it over over to your setup, you're all set! <br><br> |
Revision as of 08:30, 6 April 2010
Safety
Materials
DescriptionPiranha is a powerful mix of sulfuric acid and hydrogen peroxide, used to clean microfluidic channels and render surfaces highly hydrophilic. Since it acts as an oxidizer, it is particularly useful against organics. The idea is to mix 1 part hydrogen peroxide into 3 parts sulphuric acid. For microfluidic cleansing, you'll only require a small amount (about 4ml). Don't make any more then you need! Preparation
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