Griffitts:Bacterial Strains: Difference between revisions

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(New page: {{Griffitts}} {| border="1" cellpadding="5" cellspacing="0" class="wikitable sortable" |+'''Griffitts Lab Bacterial Strains''' |- ! style="background:#efefef;" | Strain # ! style="backgrou...)
 
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|+'''Griffitts Lab Bacterial Strains'''
|+'''Griffitts Lab Bacterial Strains'''
|-
|-
! style="background:#efefef;" | Strain #
! width="30" style="background:#efefef;" | Strain #
! style="background:#efefef;" | Parental strain
! width="50" style="background:#efefef;" | Parental strain
! style="background:#efefef;" | Plasmid
! width="50" style="background:#efefef;" | Plasmid
! width="500" style="background:#ffdead;" | Notes
! style="background:#efefef;" | Marker
! width="600" style="background:#ffdead;" | Notes
|-
|-
| B001
| B001
| DH5α (Nal<sup>R</sup>)
| DH5α
| pRK600 (Cm<sup>R</sup>)
| pRK600
| Cm<sup>R</sup>)
| pRK600 from MT616<br>
| pRK600 from MT616<br>
Demonstrated to be a functional helper strain
Demonstrated to be a functional helper strain
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| RM1021
| RM1021
| pJG107
| pJG107
| None
| pJG107 integrated at idhA locus<br>
| pJG107 integrated at idhA locus<br>
intended as a negative control but can also serve as a general integration vector
intended as a negative control but can also serve as a general integration vector
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| RM1021
| RM1021
| pJG108
| pJG108
| None
| pJG108 integrated at idhA locus<br>
| pJG108 integrated at idhA locus<br>
yeilds "screaming" GUS activity due to the trp promoter
yeilds "screaming" GUS activity due to the trp promoter
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| B004
| B004
| RM1021
| RM1021
| pJG111 transposon (Gm<sup>R</sup>)
| None
| Isolate 1
| Gm<sup>R</sup>
| Isolate 1; transposon from pJG111
|-
|-
| B005
| B005
| RM1021
| RM1021
| pJG111 transposon (Gm<sup>R</sup>)
| None
| Isolate 2
| Gm<sup>R</sup>
| Isolate 2; transposon from pJG111
|-
|-
| B006
| B006
| ''S. m.''
| ''S. m.''
|
| None
| None
| lptA (low-pH tolerant)
| lptA (low-pH tolerant)
|-
|-
| B007
| B007
| ''S. m.''
| ''S. m.''
|
| None
| None
| lptB (low-pH tolerant)
| lptB (low-pH tolerant)
|-
|-
| B008
| B008
| RM1021
| RM1021
| pJG118 transposon (Gm<sup>R</sup>)
| None
| Gm<sup>R</sup>, Sm<sup>R</sup>
| Was "M53A"<br>
| Was "M53A"<br>
Resistant on Gm<sub>25</sub>Sm<sub>250</sub>
pJG118 transposon
|-
|-
| B009
| B009
| RM1021
| RM1021
| pJG118 transposon (Gm<sup>R</sup>)
| None
| Gm<sup>R</sup>, Sm<sup>R</sup>
| Was "M53B"<br>
| Was "M53B"<br>
Resistant on Gm<sub>25</sub>Sm<sub>250</sub>
pJG118 transposon
|-
|-
| B010
| B010
| M112-1
| M112-1
| pJG112
| pJG112
| None
| cre-mediated excision of loxP-flanked DNA
| cre-mediated excision of loxP-flanked DNA
|-
|-
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| M112-2
| M112-2
| pJG112
| pJG112
| None
| cre-mediated excision of loxP-flanked DNA
| cre-mediated excision of loxP-flanked DNA
|}
|}

Revision as of 15:46, 27 September 2007


Griffitts Lab Bacterial Strains
Strain # Parental strain Plasmid Marker Notes
B001 DH5α pRK600 CmR) pRK600 from MT616

Demonstrated to be a functional helper strain

B002 RM1021 pJG107 None pJG107 integrated at idhA locus

intended as a negative control but can also serve as a general integration vector

B003 RM1021 pJG108 None pJG108 integrated at idhA locus

yeilds "screaming" GUS activity due to the trp promoter

B004 RM1021 None GmR Isolate 1; transposon from pJG111
B005 RM1021 None GmR Isolate 2; transposon from pJG111
B006 S. m. None None lptA (low-pH tolerant)
B007 S. m. None None lptB (low-pH tolerant)
B008 RM1021 None GmR, SmR Was "M53A"

pJG118 transposon

B009 RM1021 None GmR, SmR Was "M53B"

pJG118 transposon

B010 M112-1 pJG112 None cre-mediated excision of loxP-flanked DNA
B011 M112-2 pJG112 None cre-mediated excision of loxP-flanked DNA
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