Griffitts:Bacterial Strains
From OpenWetWare
| Strain # | Parental strain | Plasmid | Marker | Notes |
|---|---|---|---|---|
| B001 | DH5α | pRK600 | CmR | pRK600 from MT616 Demonstrated to be a functional helper strain |
| B024 | RM1021 | None | None | ΔSmb21188 (acyl-T) |
| B025 | RM1021 | None | None | ΔSmb21189 and ΔSmb21190 (glycosyltransferases) |
| B026 | RM1021 | None | None | ΔSmb21191 (msbA2) |
| B042 | RM1021 | pTB93F | None | GFP+ |
| B043 | DH5α | pXLGD4 | TcR | hemA::lacZ in pRK290 |
| B044 | DH5α | pTB93F | SpR | ptrp::GFP S65T in pMB393 |
| B060 | M. loti | None | FmR | MAFF303099; grows on TY-fosfomycin100 |
| B061 | XL1-Blue | pMAL-C2G | None | |
| B062 | XL1-Blue | pGEX-5X-1 | None | |
| B063 | DH5α | pGEX-4T-1 | None | |
| B068 | XL1-Blue | pDG71 | TcR, KmR | plasmid provides ptrp:GFP (mut3) on pHC41, highly stable in planta |
| B078 | MR1021 | pRF771 | None | control strain |
| B079 | MG1655 | None | None | |
| B081 | MC4100 | None | SmR | F- araD139 Δ(argf-lac)U169* rpsL150 relA1 flb5301 fruA25 deoC1 ptsF25 e14- |
| B082 | BW25139 | None | None | pir+ recA+ |
| B083 | BW25141 | None | None | pir+ recA- (use this one for cloning R6K plasmids) |
| B084 | BW2141 | pGP704 | ApR | pGP704 is pir-dependent |
| B087 | S. fredii | None | None | USDA257 |
| B088 | S. fredii | None | None | NGR234 |
| B089 | A. tumefaciens | None | None | LBA4404 |
| B090 | M. loti | None | None | NZP2037 |
| B091 | R. tropici | None | None | BR816 |
| B093 | B. japonicum | None | None | USDA110 |
| B094 | MG1655 | None | SmR | transduced with lysate from MC4100 |
| B095 | DH5α | None | None | |
| B096 | DH5α | pRF771 | None | |
| B097 | KS1000 | None | None | from NEB |
| B098 | TB1 | None | None | from NEB |
| B099 | DH5α | pJQ200SK | None | May be HB101 instead of DH5α |
| B100 | RM1021 | None | None | |
| B103 | RM1021 | None | None | expR101 (expR+ |
| B119 | RM1021 | None | None | ΔfeuP |
| B122 | RM1021 | None | None | ΔfeuQ |
| B125 | RM1021 | None | None | trsA mutant, trsA::Tn5-110 |
| B146 | RM1021 | None | None | hisH- (interupted by Tn5-110) |
| B147 | RM1021 | None | None | aroF- (interupted by Tn5-110) |
| B148 | RM1021 | None | None | carA- (interupted by Tn5-110) |
| B149 | RM1021 | None | None | ndvA- (interupted by Tn5-110) |
| B150 | RM1021 | None | None | fixB- (interupted by Tn5-110) |
| B151 | RM1021 | None | None | dme- (interupted by Tn5-110) |
| B152 | RM1021 | None | None | fixI- (interupted by Tn5-110) |
| B154 | RM1021 | None | None | exoB- (interupted by Tn5-110) |
| B155 | RM1021 | None | None | nifK- (interupted by Tn5-110) |
| B156 | RM1021 | None | None | thrB- (interupted by Tn5-110) |
| B157 | RM1021 | None | None | tkt2- (interupted by Tn5-110) |
| B158 | RM1021 | None | None | ccmB- (interupted by Tn5-110) |
| B159 | RM1021 | None | None | exoF- (interupted by Tn5-110) |
| B160 | RM1021 | None | None | pckA- (interupted by Tn5-110) |
| B161 | RM1021 | None | None | carB- (interupted by Tn5-110) |
| B162 | RM1021 | None | None | relA- (interupted by Tn5-110) |
| B163 | RM1021 | None | None | bacA- (interupted by Tn5-110) |
| B164 | RM1021 | None | None | cycJ- (interupted by Tn5-110) |
| B165 | RM1021 | None | None | unknown gene essential for symbiosis interupted by Tn5-110 |
| B166 | RM1021 | None | None | dctA- (interupted by Tn5-110) |
| B167 | RM1021 | None | None | phaA1- (interupted by Tn5-110) |
| B168 | RM1021 | None | None | ilvD2- (interupted by Tn5-110) |
| B169 | RM1021 | None | None | nodH- (interupted by Tn5-110) |
| B170 | RM1021 | None | None | leuD- (interupted by Tn5-110) |
| B171 | RM1021 | None | None | exoY- (interupted by Tn5-110) |
| B172 | RM1021 | None | None | fixG- (interupted by Tn5-110) |
| B173 | RM1021 | None | None | leuB- (interupted by Tn5-110) |
| B174 | RM1021 | None | None | dctB- (interupted by Tn5-110) |
| B175 | RM1021 | None | None | phoC- (interupted by Tn5-110) |
| B176 | RM1021 | None | None | phoE- (interupted by Tn5-110) |
| B177 | RM1021 | None | None | gltD- (interupted by Tn5-110) |
| B178 | RM1021 | None | None | ccmC- (interupted by Tn5-110) |
| B179 | RM1021 | None | None | purF- (interupted by Tn5-110) |
| B180 | RM1021 | None | None | unknown gene essential for symbiosis interupted by Tn5-110 |
| B181 | RM1021 | None | None | hisF- (interupted by Tn5-110) |
| B181 | RM1021 | None | None | hisF- (interupted by Tn5-110) |
| B181 | RM1021 | None | None | hisF- (interupted by Tn5-110) |
| B182 | RM1021 | None | None | cycH- (interupted by Tn5-110) |
| B183 | RM1021 | None | None | leuC- (interupted by Tn5-110) |
| B184 | RM1021 | None | None | leuA- (interupted by Tn5-110) |
| B185 | RM1021 | None | None | nifB- (interupted by Tn5-110) |
| B186 | RM1021 | None | None | fixX- (interupted by Tn5-110) |
| B187 | RM1021 | None | None | feuQ- (interupted by Tn5-110) |
| B188 | RM1021 | None | None | feuP- (interupted by Tn5-110) |
| B189 | RM1021 | None | None | fixL- (interupted by Tn5-110) |
| B190 | RM1021 | None | None | trpE- (interupted by Tn5-110) |
| B191 | RM1021 | None | None | purK- (interupted by Tn5-110) |
| B192 | RM1021 | None | None | purL- (interupted by Tn5-110) |
| B193 | RM1021 | None | None | purC- (interupted by Tn5-110) |
