Griffitts:Common buffers

Electrophoresis Buffers

8X DNA loading

• 40% glycerol
• 1/50 dilution of bromophenol blue

6X SDS loading

• 7.5 mL 80% glycerol
• 2.5 mL 1M Tris pH 6.8
• 1.2 g SDS
• 600 μL 2-mercaptoethanol
• 150 μL 2% bromophenol blue

2% bromophenol blue

• 0.4 g BPB
• 20 mL 50 mM Tris pH 8.0
  

Sterile filter

DNA ladder

• 147 μL ddH₂0
• 34 μL 8X DNA loading buffer
• 20 μL 1 kb ladder from NEB
• 2 μL 0.25 M EDTA
  

Note: use 5 μL per lane

ethidium bromide (10 mL)

• 10 mL ddH₂0
• 10 mg ethidium bromide
  

Note: use 8 μL per 50 mL gel

50X TAE (1 L)

• ~800 mL ddH₂0
• 242 g Trizma base
• 57.1 mL glacial acetic acid
• 100 mL 0.25 M EDTA (pH 8.0)
  

QS to volume with ddH₂0
Note: this is half the amount of EDTA compared to standard TAE