Griffitts:DNA sequencing: Difference between revisions
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==DNA Prep== | ==DNA Prep== | ||
* Make a 1:10 dilution of each of your primers | * Make a 1:10 dilution of each of your primers | ||
Revision as of 10:07, 11 April 2008
DNA Prep
- Make a 1:10 dilution of each of your primers
- 3 μL primer + 27 μL ddH20 will usually suffice
- Label your tubes
- Use 1.5 mL microcentrifuge tubes
- Add 1 μL of the appropriate primer to each tube
- Add 9 μL DNA from a cleaned up PCR
- Each sequence will require two samples—one with the upstream primer and one with the downstream primer
- Report your sequencing reactions to Dr. Griffitts or online
- Take your sequencing reactions to 690 WIDB and put them in the refrigerator
- Make sure the liquid is at the bottom of the tube
