Griffitts:DNA sequencing

DNA Prep

• Make a 1:10 dilution of each of your primers
  • 3 μL primer + 27 μL ddH₂0 will usually suffice
• Label your tubes
  • Use 1.5 mL microcentrifuge tubes
• Add 1 μL of the appropriate primer to each tube
• Add 9 μL DNA from a cleaned up PCR
  • Each sequence will require two samples—one with the upstream primer and one with the downstream primer
• Report your sequencing reactions to Dr. Griffitts or online
• Take your sequencing reactions to 690 WIDB and put them in the refrigerator
  • Make sure the liquid is at the bottom of the tube