Griffitts:DNAse treatment

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Procedure

• Resuspend pellet in a mixture of 45 μL ddH₂O and 5 μL NEB DNase buffer
• Add 1 μL of DNAse I (in freezer; keep on ice)
• Mix thoroughly
• Place in 37°C water bath for 10 minutes
• Add 2 μL of 0.25 M EDTA
• Heat at 75°C for 10 minutes
• Store sample on ice or in -20°C freezer.

When ready proceed to Reverse transcription