Griffitts:Gel recipes

From OpenWetWare

Revision as of 15:56, 11 April 2008 by Matthew B. Crook (Talk | contribs)
(diff) ←Older revision | Current revision (diff) | Newer revision→ (diff)
Jump to: navigation, search

Contents

Denaturing gels

Resolving gel (15%) (5 mL)

Resolving gel (10%) (5 mL)

Stacking gel (4%) (3 mL)

Native gels

Native Resolving gel (10%) (5mL)

Native Stacking gel (4%) (3mL)

Notes

  • This protocol will work only for proteins with acidic pKi (4–6.8)
  • This protocol is the same as SDS-PAGE but with no SDS, and different sample buffer
  • Do not boil sample
  • 2-mercaptoethanol may be omitted from sample buffer
Personal tools