Griffitts:Plasmids

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Griffitts Lab Bacterial Strains
Plasmid # Parental plasmid Marker Notes
pJG245 pJG210, pJG199 NmR In B083; promoterless lacZ
pJG246 pJG210, pJG199 NmR In B083; same as pJG245 but without promoterless lacZ
pJG247 None NmR pir-dependent; for E. coli loop-ins
pJG249 pMAL-c2g None Perfect clone of pHISc2
pJG250 pJG181, pJG194 None GUS from pJG181 cloned into pJG194
pJG255 pHISc2 ApR feuPWT cloned with EcoR1/Xba
pJG256 pMAC-c2g ApR feuPWT cloned with EcoR1/Xba
pJG259 pRF771 None Gives lowest level of transcription from EcoR1 side
pJG263 pJG259 None lacZ cloned into BamH1/Xba; Standard lacZ vector
pJG265 pJG259 None GUS cloned into BamH1/Xba
pJG267 pJG109 TcR TcR from pJG151 replaced NmR with EcoR1/BamH1
pJG271 pJG263 TcR PtauA::lacZ
pJG280 pJG263 TcR mel promoter fragment (lacZ) cloned into EcoR1/Xba
pJG285 pJG246 TcR In B083; TcR from pJG267 replaced KmR with EcoR1/BamH1
pJG286 pJG263 None PndvA cloned into EcoR1/Xba to make a lacZ fusion
pJG288 pKD46, pJG109 KmR SC101TS from pKD46 and KmR-tnp from pJG109 were digested with AurII and ligated together
pJG289 pKD46, pJG267 TcR SC101TS from pKD46 and tetA/R-tnp from pJG267 were digested with AurII and ligated together
pJG293 pJG202, pJG271 None feuP from pJG202 cloned into Xba/BamH1 of pJG271; feuP expression should be taurine-inducible
pJG296 pHISc2 None D51E mutation of feuP cloned with EcoR1/Xba
pJG297 pHISc2 None D51A mutation of feuP cloned with EcoR1/Xba
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