Griffitts:Protocols

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{{Template:Griffitts}}
{{Template:Griffitts}}
==DNA==
==DNA==
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*Qiagen Miniprep
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*[[Griffitts:Miniprep|Qiagen Miniprep]]
*Taq PCR
*Taq PCR
*Pfx PCR
*Pfx PCR

Revision as of 19:08, 14 August 2007

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Contents

DNA

  • Qiagen Miniprep
  • Taq PCR
  • Pfx PCR
  • Overlap-extension PCR
  • Arbitrary PCR
  • Restriction Digest
  • Low-melt Gel Purification
  • In-gel Ligation

RNA

  • Trizol(Bacterial)
  • DNAse treatment
  • Reverse transcription
  • 5'RACE

Protein

  • Gel recipes
  • Sample buffer
  • Bacterial lysis
  • 6His purification (6His)
  • MBP purification (MBP)

Bacteria

  • Media
  • Freezing Procedure
  • DNA prep
  • RNA prep
  • Triparental Mating
  • Transduction
  • Tips on streaking

Plants

  • Seed scarification
  • Media
  • DNA prep
  • RNA prep
  • Seed Storage
  • Microscopy
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