Griffitts:Protocols
From OpenWetWare
(Difference between revisions)
| Line 1: | Line 1: | ||
{{Template:Griffitts}} | {{Template:Griffitts}} | ||
| + | ==Recipes== | ||
| + | *Common buffers | ||
| + | *Common media | ||
==DNA== | ==DNA== | ||
*[[Griffitts:Miniprep|Qiagen Miniprep]] | *[[Griffitts:Miniprep|Qiagen Miniprep]] | ||
| + | *Low-copy plasmid prep | ||
*[[Griffitts:TaqPCR|Taq PCR]] | *[[Griffitts:TaqPCR|Taq PCR]] | ||
*Pfx PCR | *Pfx PCR | ||
*Overlap-extension PCR | *Overlap-extension PCR | ||
*Arbitrary PCR | *Arbitrary PCR | ||
| - | *Restriction | + | *Restriction digest |
| - | + | *In-gel ligation | |
| - | *In-gel | + | |
==RNA== | ==RNA== | ||
| - | *Trizol( | + | *Trizol purification (bacterial) |
*DNAse treatment | *DNAse treatment | ||
*Reverse transcription | *Reverse transcription | ||
| Line 22: | Line 25: | ||
==Bacteria== | ==Bacteria== | ||
*Media | *Media | ||
| + | *Tips on streaking/culturing | ||
*Freezing Procedure | *Freezing Procedure | ||
| - | *DNA prep | + | *Genomic DNA prep |
| - | *RNA prep | + | *RNA prep (Trizol) |
*Triparental Mating | *Triparental Mating | ||
*Transduction | *Transduction | ||
| - | |||
==Plants== | ==Plants== | ||
| - | |||
*Media | *Media | ||
| + | *Seed scarification | ||
| + | *Tips for growth/seed storage | ||
*DNA prep | *DNA prep | ||
*RNA prep | *RNA prep | ||
*Seed Storage | *Seed Storage | ||
*Microscopy | *Microscopy | ||
Revision as of 11:13, 16 August 2007
Home Research Lab Members Protocols Publications Internal
Contents |
Recipes
- Common buffers
- Common media
DNA
- Qiagen Miniprep
- Low-copy plasmid prep
- Taq PCR
- Pfx PCR
- Overlap-extension PCR
- Arbitrary PCR
- Restriction digest
- In-gel ligation
RNA
- Trizol purification (bacterial)
- DNAse treatment
- Reverse transcription
- 5'RACE
Protein
- Gel recipes
- Sample buffer
- Bacterial lysis
- 6His purification (6His)
- MBP purification (MBP)
Bacteria
- Media
- Tips on streaking/culturing
- Freezing Procedure
- Genomic DNA prep
- RNA prep (Trizol)
- Triparental Mating
- Transduction
Plants
- Media
- Seed scarification
- Tips for growth/seed storage
- DNA prep
- RNA prep
- Seed Storage
- Microscopy
