Griffitts:Protocols

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{{Template:Griffitts}}
{{Template:Griffitts}}
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==Recipes==
 +
*Common buffers
 +
*Common media
==DNA==
==DNA==
*[[Griffitts:Miniprep|Qiagen Miniprep]]
*[[Griffitts:Miniprep|Qiagen Miniprep]]
 +
*Low-copy plasmid prep
*[[Griffitts:TaqPCR|Taq PCR]]
*[[Griffitts:TaqPCR|Taq PCR]]
*Pfx PCR
*Pfx PCR
*Overlap-extension PCR
*Overlap-extension PCR
*Arbitrary PCR
*Arbitrary PCR
-
*Restriction Digest
+
*Restriction digest
-
*Low-melt Gel Purification
+
*In-gel ligation
-
*In-gel Ligation
+
==RNA==
==RNA==
-
*Trizol(Bacterial)
+
*Trizol purification (bacterial)
*DNAse treatment
*DNAse treatment
*Reverse transcription
*Reverse transcription
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==Bacteria==
==Bacteria==
*Media
*Media
 +
*Tips on streaking/culturing
*Freezing Procedure
*Freezing Procedure
-
*DNA prep
+
*Genomic DNA prep
-
*RNA prep
+
*RNA prep (Trizol)
*Triparental Mating
*Triparental Mating
*Transduction
*Transduction
-
*Tips on streaking
 
==Plants==
==Plants==
-
*Seed scarification
 
*Media
*Media
 +
*Seed scarification
 +
*Tips for growth/seed storage
*DNA prep
*DNA prep
*RNA prep
*RNA prep
*Seed Storage
*Seed Storage
*Microscopy
*Microscopy

Revision as of 10:13, 16 August 2007

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Contents

Recipes

  • Common buffers
  • Common media

DNA

  • Qiagen Miniprep
  • Low-copy plasmid prep
  • Taq PCR
  • Pfx PCR
  • Overlap-extension PCR
  • Arbitrary PCR
  • Restriction digest
  • In-gel ligation

RNA

  • Trizol purification (bacterial)
  • DNAse treatment
  • Reverse transcription
  • 5'RACE

Protein

  • Gel recipes
  • Sample buffer
  • Bacterial lysis
  • 6His purification (6His)
  • MBP purification (MBP)

Bacteria

  • Media
  • Tips on streaking/culturing
  • Freezing Procedure
  • Genomic DNA prep
  • RNA prep (Trizol)
  • Triparental Mating
  • Transduction

Plants

  • Media
  • Seed scarification
  • Tips for growth/seed storage
  • DNA prep
  • RNA prep
  • Seed Storage
  • Microscopy
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