Griffitts:Protocols

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*Genomic DNA prep
*Genomic DNA prep
*RNA prep (Trizol)
*RNA prep (Trizol)
 +
*Beta-gal/gus assays
*Triparental Mating
*Triparental Mating
*Transduction
*Transduction

Revision as of 11:15, 16 August 2007

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Contents

Recipes

  • Common buffers
  • Common media

DNA

  • Qiagen Miniprep
  • Low-copy plasmid prep
  • Taq PCR
  • Pfx PCR
  • Overlap-extension PCR
  • Arbitrary PCR
  • Restriction digest
  • In-gel ligation

RNA

  • Trizol purification (bacterial)
  • DNAse treatment
  • Reverse transcription
  • 5'RACE

Protein

  • Gel recipes
  • Sample buffer
  • Bacterial lysis
  • 6His purification (6His)
  • MBP purification (MBP)

Bacteria

  • Media
  • Antibiotics
  • Tips on streaking/culturing
  • Freezing Procedure
  • Genomic DNA prep
  • RNA prep (Trizol)
  • Beta-gal/gus assays
  • Triparental Mating
  • Transduction

Plants

  • Media
  • Seed scarification
  • Tips for growth/seed storage
  • DNA prep
  • RNA prep
  • Seed Storage
  • Microscopy
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