Griffitts:Protocols: Difference between revisions
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*Genomic DNA prep | *Genomic DNA prep | ||
*RNA prep (Trizol) | *RNA prep (Trizol) | ||
*Beta-gal/gus assays | |||
*Triparental Mating | *Triparental Mating | ||
*Transduction | *Transduction | ||
Revision as of 08:15, 16 August 2007
Recipes
- Common buffers
- Common media
DNA
- Qiagen Miniprep
- Low-copy plasmid prep
- Taq PCR
- Pfx PCR
- Overlap-extension PCR
- Arbitrary PCR
- Restriction digest
- In-gel ligation
RNA
- Trizol purification (bacterial)
- DNAse treatment
- Reverse transcription
- 5'RACE
Protein
- Gel recipes
- Sample buffer
- Bacterial lysis
- 6His purification (6His)
- MBP purification (MBP)
Bacteria
- Media
- Antibiotics
- Tips on streaking/culturing
- Freezing Procedure
- Genomic DNA prep
- RNA prep (Trizol)
- Beta-gal/gus assays
- Triparental Mating
- Transduction
Plants
- Media
- Seed scarification
- Tips for growth/seed storage
- DNA prep
- RNA prep
- Seed Storage
- Microscopy
