Griffitts:Protocols
From OpenWetWare
(Difference between revisions)
(→Recipes: Added Red) |
|||
| Line 1: | Line 1: | ||
{{Template:Griffitts}} | {{Template:Griffitts}} | ||
==Recipes== | ==Recipes== | ||
| - | *Common buffers | + | *[[Griffitts:Common buffers|Common buffers]] |
| - | *Common media | + | *[[Griffitts:Common media|Common media]] |
| + | |||
==DNA== | ==DNA== | ||
*[[Griffitts:Miniprep|Qiagen Miniprep]] | *[[Griffitts:Miniprep|Qiagen Miniprep]] | ||
Revision as of 16:15, 7 September 2007
Home Research Lab Members Protocols Publications Internal
Contents |
Recipes
DNA
- Qiagen Miniprep
- Low-copy plasmid prep
- Taq PCR
- Pfx PCR
- Overlap-extension PCR
- Arbitrary PCR
- Restriction digest
- In-gel ligation
RNA
- Trizol purification (bacterial)
- DNAse treatment
- Reverse transcription
- 5'RACE
Protein
- Gel recipes
- Sample buffer
- Bacterial lysis
- 6His purification (6His)
- MBP purification (MBP)
Bacteria
- Media
- Antibiotics
- Tips on streaking/culturing
- Freezing Procedure
- Genomic DNA prep
- RNA prep (Trizol)
- Beta-gal/gus assays
- Triparental Mating
- Transduction
Plants
- Media
- Seed scarification
- Tips for growth/seed storage
- DNA prep
- RNA prep
- Seed Storage
- Microscopy
