Griffitts:Protocols

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(Plants)
(Protein)
Line 19: Line 19:
*5'RACE
*5'RACE
==Protein==
==Protein==
-
*Gel recipes
+
*[[Gel recipes]]
*Sample buffer
*Sample buffer
*Bacterial lysis
*Bacterial lysis
*6His purification (6His)
*6His purification (6His)
*MBP purification (MBP)
*MBP purification (MBP)
 +
==Bacteria==
==Bacteria==
*[[Griffitts:Common media#Bacterial Media and Additives|Media]]
*[[Griffitts:Common media#Bacterial Media and Additives|Media]]

Revision as of 14:11, 8 September 2007

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Contents

Recipes

DNA

  • Qiagen Miniprep
  • Low-copy plasmid prep
  • Taq PCR
  • Pfx PCR
  • Overlap-extension PCR
  • Arbitrary PCR
  • Restriction digest
  • In-gel ligation

RNA

  • Trizol purification (bacterial)
  • DNAse treatment
  • Reverse transcription
  • 5'RACE

Protein

  • Gel recipes
  • Sample buffer
  • Bacterial lysis
  • 6His purification (6His)
  • MBP purification (MBP)

Bacteria

  • Media
  • Antibiotics
  • Tips on streaking/culturing
  • Freezing Procedure
  • Genomic DNA prep
  • RNA prep (Trizol)
  • Beta-gal/gus assays
  • Triparental Mating
  • Transduction

Plants

  • Media
  • Seed scarification
  • Tips for growth/seed storage
  • DNA prep
  • RNA prep
  • Seed Storage
  • Microscopy
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