Griffitts:Protocols

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(Protein)
(Protein)
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*5'RACE
*5'RACE
==Protein==
==Protein==
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*[[Gel recipes]]
+
*[[Griffitts:Gel recipes]]
*Sample buffer
*Sample buffer
*Bacterial lysis
*Bacterial lysis

Revision as of 14:21, 8 September 2007

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Contents

Recipes

DNA

  • Qiagen Miniprep
  • Low-copy plasmid prep
  • Taq PCR
  • Pfx PCR
  • Overlap-extension PCR
  • Arbitrary PCR
  • Restriction digest
  • In-gel ligation

RNA

  • Trizol purification (bacterial)
  • DNAse treatment
  • Reverse transcription
  • 5'RACE

Protein

Bacteria

  • Media
  • Antibiotics
  • Tips on streaking/culturing
  • Freezing Procedure
  • Genomic DNA prep
  • RNA prep (Trizol)
  • Beta-gal/gus assays
  • Triparental Mating
  • Transduction

Plants

  • Media
  • Seed scarification
  • Tips for growth/seed storage
  • DNA prep
  • RNA prep
  • Seed Storage
  • Microscopy
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