Griffitts:Protocols

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m (Bacteria)
Line 7: Line 7:
*[[Griffitts:Miniprep|Qiagen Miniprep]]
*[[Griffitts:Miniprep|Qiagen Miniprep]]
*Low-copy plasmid prep
*Low-copy plasmid prep
-
*[[Griffitts:TaqPCR|Taq PCR]]
+
*[[Griffitts:TaqPCR|Standard PCR]]
*Pfx PCR
*Pfx PCR
*Overlap-extension PCR
*Overlap-extension PCR

Revision as of 13:36, 2 October 2007

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Contents

Recipes

DNA

RNA

  • Trizol purification (bacterial)
  • DNAse treatment
  • Reverse transcription
  • 5'RACE

Protein

  • Gel recipes
  • Sample buffer
  • Bacterial lysis
  • 6His purification (6His)
  • MBP purification (MBP)

Bacteria

  • Media
  • Antibiotics
  • Tips on streaking/culturing
  • Freezing Procedure
  • Genomic DNA prep
  • RNA prep (Trizol)
  • ß-Gal/GUS assay
  • Triparental Mating
  • Transduction

Plants

  • Media
  • Seed scarification
  • Tips for growth/seed storage
  • DNA prep
  • RNA prep
  • Seed Storage
  • Microscopy
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