Griffitts:Protocols

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(Recipes)
(DNA)
Line 6: Line 6:
==DNA==
==DNA==
-
*[[Griffitts:Miniprep|Qiagen Miniprep]]
+
* [[Griffitts:Miniprep|Qiagen Miniprep]]
-
*Low-copy plasmid prep
+
* [[Griffitts:Low-copy plasmid prep|Low-copy plasmid prep]]
-
*[[Griffitts:TaqPCR|Standard PCR]]
+
* [[Griffitts:TaqPCR|Standard PCR]]
-
*Pfx PCR
+
* [[Griffitts:Pfx PCR|Pfx PCR]]
-
*Overlap-extension PCR
+
* [[Griffitts:Overlap-extension PCR|Overlap-extension PCR]]
-
*Arbitrary PCR
+
* [[Griffitts:Arbitrary PCR|Arbitrary PCR]]
-
*Restriction digest
+
* [[Griffitts:Restriction digest|Restriction digest]]
-
*In-gel ligation
+
* [[Griffitts:In-gel ligation|In-gel ligation]]
 +
 
==RNA==
==RNA==
*Trizol purification (bacterial)
*Trizol purification (bacterial)

Revision as of 18:32, 1 November 2007

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Contents

Recipes

DNA

RNA

  • Trizol purification (bacterial)
  • DNAse treatment
  • Reverse transcription
  • 5'RACE

Protein

  • Gel recipes
  • Sample buffer
  • Bacterial lysis
  • 6His purification (6His)
  • MBP purification (MBP)

Bacteria

  • Media
  • Antibiotics
  • Tips on streaking/culturing
  • Freezing Procedure
  • Genomic DNA prep
  • RNA prep (Trizol)
  • ß-Gal/GUS assay
  • Triparental Mating
  • Transduction

Plants

Personal tools