Griffitts:Protocols

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m (Plants)
(DNA)
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* [[Griffitts:Overlap-extension PCR|Overlap-extension PCR]]
* [[Griffitts:Overlap-extension PCR|Overlap-extension PCR]]
* [[Griffitts:Arbitrary PCR|Arbitrary PCR]]
* [[Griffitts:Arbitrary PCR|Arbitrary PCR]]
 +
* [[Griffitts:PCR clean-up|PCR clean-up (Qiagen)]]
* [[Griffitts:Restriction digest|Restriction digest]]
* [[Griffitts:Restriction digest|Restriction digest]]
* [[Griffitts:In-gel ligation|In-gel ligation]]
* [[Griffitts:In-gel ligation|In-gel ligation]]

Revision as of 11:27, 2 November 2007

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Contents

Recipes

DNA

RNA

  • Trizol purification (bacterial)
  • DNAse treatment
  • Reverse transcription
  • 5'RACE

Protein

  • Gel recipes
  • Sample buffer
  • Bacterial lysis
  • 6His purification (6His)
  • MBP purification (MBP)

Bacteria

  • Media
  • Antibiotics
  • Tips on streaking/culturing
  • Freezing Procedure
  • Genomic DNA prep
  • RNA prep (Trizol)
  • ß-Gal/GUS assay
  • Triparental Mating
  • Transduction

Plants

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