Griffitts:Protocols: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
m (→Plants) |
(→DNA) |
||
| Line 12: | Line 12: | ||
* [[Griffitts:Overlap-extension PCR|Overlap-extension PCR]] | * [[Griffitts:Overlap-extension PCR|Overlap-extension PCR]] | ||
* [[Griffitts:Arbitrary PCR|Arbitrary PCR]] | * [[Griffitts:Arbitrary PCR|Arbitrary PCR]] | ||
* [[Griffitts:PCR clean-up|PCR clean-up (Qiagen)]] | |||
* [[Griffitts:Restriction digest|Restriction digest]] | * [[Griffitts:Restriction digest|Restriction digest]] | ||
* [[Griffitts:In-gel ligation|In-gel ligation]] | * [[Griffitts:In-gel ligation|In-gel ligation]] | ||
Revision as of 08:27, 2 November 2007
Recipes
DNA
- Qiagen Miniprep
- Low-copy plasmid prep
- Standard PCR
- Pfx PCR
- Overlap-extension PCR
- Arbitrary PCR
- PCR clean-up (Qiagen)
- Restriction digest
- In-gel ligation
RNA
- Trizol purification (bacterial)
- DNAse treatment
- Reverse transcription
- 5'RACE
Protein
- Gel recipes
- Sample buffer
- Bacterial lysis
- 6His purification (6His)
- MBP purification (MBP)
Bacteria
- Media
- Antibiotics
- Tips on streaking/culturing
- Freezing Procedure
- Genomic DNA prep
- RNA prep (Trizol)
- ß-Gal/GUS assay
- Triparental Mating
- Transduction
