Griffitts:Protocols

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==Bacteria==
==Bacteria==
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*[[Griffitts:Common media#Bacterial Media and Additives|Media]]
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* [[Griffitts:Common media#Bacterial Media and Additives|Media]]
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*Antibiotics
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* [[Griffitss:Stock_solutions#Antibiotics|Antibiotics]]
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*Tips on streaking/culturing
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* Tips on streaking/culturing
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*Freezing Procedure
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* Freezing Procedure
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*Genomic DNA prep
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* Genomic DNA prep
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*RNA prep (Trizol)
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* RNA prep (Trizol)
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*[[Griffitts:ß-Gal/GUS assay|ß-Gal/GUS assay]]
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* [[Griffitts:ß-Gal/GUS assay|ß-Gal/GUS assay]]
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*Triparental Mating
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* Triparental Mating
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*Transduction
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* Transduction
==Plants==
==Plants==

Revision as of 13:37, 6 November 2007

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Contents

Recipes

DNA

RNA

  • Trizol purification (bacterial)
  • DNAse treatment
  • Reverse transcription
  • 5'RACE

Protein

  • Gel recipes
  • Sample buffer
  • Bacterial lysis
  • 6His purification (6His)
  • MBP purification (MBP)

Bacteria

Plants

Personal tools