Griffitts:Protocols

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(Bacteria)
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==Bacteria==
==Bacteria==
* [[Griffitts:Common media#Bacterial Media and Additives|Media]]
* [[Griffitts:Common media#Bacterial Media and Additives|Media]]
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* [[Griffitss:Stock_solutions#Antibiotics|Antibiotics]]
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* [[Griffitts:Stock_solutions#Antibiotics|Antibiotics]]
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* Tips on streaking/culturing
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* [[Griffitts:Culture tips|Tips on streaking/culturing]]
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* Freezing Procedure
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* [[Griffitts:Freezing bacteria|Freezing procedure]]
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* Genomic DNA prep
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* [[Griffitts:Bacterial DNA prep|Genomic DNA prep]]
* RNA prep (Trizol)
* RNA prep (Trizol)
* [[Griffitts:ß-Gal/GUS assay|ß-Gal/GUS assay]]
* [[Griffitts:ß-Gal/GUS assay|ß-Gal/GUS assay]]
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* Triparental Mating
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* [[Griffitts:Triparental Mating|Triparental Mating]]
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* Transduction
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* [[Griffitts:Transduction|Transduction]]
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* [[Griffitts:Competent Cells|Competent Cells]]
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* [[Griffitts:Electroporation|Electroporation]]
==Plants==
==Plants==

Revision as of 16:04, 6 November 2007

Home     Research     Lab Members     Protocols     Publications     Internal    


Contents

Recipes

DNA

RNA

  • Trizol purification (bacterial)
  • DNAse treatment
  • Reverse transcription
  • 5'RACE

Protein

  • Gel recipes
  • Sample buffer
  • Bacterial lysis
  • 6His purification (6His)
  • MBP purification (MBP)

Bacteria

Plants

Personal tools