Griffitts:Protocols: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
m (→Bacteria) |
|||
| Line 31: | Line 31: | ||
==Bacteria== | ==Bacteria== | ||
* [[Griffitts:Common media#Bacterial Media and Additives|Media]] | * [[Griffitts:Common media#Bacterial Media and Additives|Media]] | ||
* [[ | * [[Griffitts:Stock_solutions#Antibiotics|Antibiotics]] | ||
* Tips on streaking/culturing | * [[Griffitts:Culture tips|Tips on streaking/culturing]] | ||
* Freezing | * [[Griffitts:Freezing bacteria|Freezing procedure]] | ||
* Genomic DNA prep | * [[Griffitts:Bacterial DNA prep|Genomic DNA prep]] | ||
* RNA prep (Trizol) | * RNA prep (Trizol) | ||
* [[Griffitts:ß-Gal/GUS assay|ß-Gal/GUS assay]] | * [[Griffitts:ß-Gal/GUS assay|ß-Gal/GUS assay]] | ||
* Triparental Mating | * [[Griffitts:Triparental Mating|Triparental Mating]] | ||
* Transduction | * [[Griffitts:Transduction|Transduction]] | ||
* [[Griffitts:Competent Cells|Competent Cells]] | |||
* [[Griffitts:Electroporation|Electroporation]] | |||
==Plants== | ==Plants== | ||
Revision as of 13:04, 6 November 2007
Recipes
DNA
- Qiagen Miniprep
- Low-copy plasmid prep
- Standard PCR
- Pfx PCR
- Overlap-extension PCR
- Arbitrary PCR
- PCR clean-up (Qiagen)
- Gel electrophoresis
- Restriction digest
- In-gel ligation
RNA
- Trizol purification (bacterial)
- DNAse treatment
- Reverse transcription
- 5'RACE
Protein
- Gel recipes
- Sample buffer
- Bacterial lysis
- 6His purification (6His)
- MBP purification (MBP)
Bacteria
- Media
- Antibiotics
- Tips on streaking/culturing
- Freezing procedure
- Genomic DNA prep
- RNA prep (Trizol)
- ß-Gal/GUS assay
- Triparental Mating
- Transduction
- Competent Cells
- Electroporation
