Griffitts:Protocols

From OpenWetWare

(Difference between revisions)
Jump to: navigation, search
(Recipes)
(Bacteria)
Line 35: Line 35:
* [[Griffitts:Freezing bacteria|Freezing procedure]]
* [[Griffitts:Freezing bacteria|Freezing procedure]]
* [[Griffitts:Bacterial DNA prep|Genomic DNA prep]]
* [[Griffitts:Bacterial DNA prep|Genomic DNA prep]]
-
* RNA prep (Trizol)
+
* [[Griffitts:Bacterial RNA prep|RNA prep (Trizol)]]
* [[Griffitts:ß-Gal/GUS assay|ß-Gal/GUS assay]]
* [[Griffitts:ß-Gal/GUS assay|ß-Gal/GUS assay]]
* [[Griffitts:Triparental Mating|Triparental Mating]]
* [[Griffitts:Triparental Mating|Triparental Mating]]

Revision as of 16:05, 6 November 2007

Home     Research     Lab Members     Protocols     Publications     Internal    


Contents

Recipes

DNA

RNA

  • Trizol purification (bacterial)
  • DNAse treatment
  • Reverse transcription
  • 5'RACE

Protein

  • Gel recipes
  • Sample buffer
  • Bacterial lysis
  • 6His purification (6His)
  • MBP purification (MBP)

Bacteria

Plants

Personal tools