Griffitts:Protocols: Difference between revisions
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* [[Griffitts:Culture tips|Tips on streaking/culturing]] | * [[Griffitts:Culture tips|Tips on streaking/culturing]] | ||
* [[Griffitts:Freezing bacteria|Freezing procedure]] | * [[Griffitts:Freezing bacteria|Freezing procedure]] | ||
* [[Griffitts:Bacterial DNA | * [[Griffitts:Bacterial DNA preparation|Genomic DNA prep]] | ||
* [[Griffitts:Bacterial RNA | * [[Griffitts:Bacterial RNA preparation|RNA prep (Trizol)]] | ||
* [[Griffitts:ß-Gal/GUS assay|ß-Gal/GUS assay]] | * [[Griffitts:ß-Gal/GUS assay|ß-Gal/GUS assay]] | ||
* [[Griffitts:Triparental Mating|Triparental Mating]] | * [[Griffitts:Triparental Mating|Triparental Mating]] | ||
Revision as of 13:06, 6 November 2007
Recipes
DNA
- Qiagen Miniprep
- Low-copy plasmid prep
- Standard PCR
- Pfx PCR
- Overlap-extension PCR
- Arbitrary PCR
- PCR clean-up (Qiagen)
- Gel electrophoresis
- Restriction digest
- In-gel ligation
RNA
- Trizol purification (bacterial)
- DNAse treatment
- Reverse transcription
- 5'RACE
Protein
- Gel recipes
- Sample buffer
- Bacterial lysis
- 6His purification (6His)
- MBP purification (MBP)
Bacteria
- Media
- Antibiotics
- Tips on streaking/culturing
- Freezing procedure
- Genomic DNA prep
- RNA prep (Trizol)
- ß-Gal/GUS assay
- Triparental Mating
- Transduction
- Competent Cells
- Electroporation
