Griffitts:Protocols

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m (DNA)
m (DNA)
Line 8: Line 8:
* [[Griffitts:Miniprep|Qiagen Miniprep]]
* [[Griffitts:Miniprep|Qiagen Miniprep]]
* [[Griffitts:Low-copy plasmid prep|Low-copy plasmid prep]]
* [[Griffitts:Low-copy plasmid prep|Low-copy plasmid prep]]
 +
* [[Griffitts:PCR primers|PCR primers]]
* [[Griffitts:TaqPCR|Standard PCR]]
* [[Griffitts:TaqPCR|Standard PCR]]
* [[Griffitts:Pfx PCR|Pfx PCR]]
* [[Griffitts:Pfx PCR|Pfx PCR]]

Revision as of 11:31, 15 November 2007

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Contents

Recipes

DNA

RNA

  • Trizol purification (bacterial)
  • DNAse treatment
  • Reverse transcription
  • 5'RACE

Protein

  • Gel recipes
  • Sample buffer
  • Bacterial lysis
  • 6His purification (6His)
  • MBP purification (MBP)

Bacteria

Plants

Personal tools