Griffitts:Protocols

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m (Plants)
m (DNA)
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==DNA==
==DNA==
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* [[Griffitts:Miniprep|Qiagen Miniprep]]
+
===PCR===
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* [[Griffitts:Low-copy plasmid prep|Low-copy plasmid prep]]
+
* [[Griffitts:PCR primers|PCR primers]]
* [[Griffitts:PCR primers|PCR primers]]
* [[Griffitts:TaqPCR|Standard PCR]]
* [[Griffitts:TaqPCR|Standard PCR]]
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* [[Griffitts:Arbitrary PCR|Arbitrary PCR]]
* [[Griffitts:Arbitrary PCR|Arbitrary PCR]]
* [[Griffitts:PCR clean-up|PCR clean-up (Qiagen)]]
* [[Griffitts:PCR clean-up|PCR clean-up (Qiagen)]]
 +
* [[Griffitts:DNA sequencing|Sequencing prep]]
 +
===DNA prep===
 +
* [[Griffitts:Miniprep|Qiagen Miniprep]]
 +
* [[Griffitts:Low-copy plasmid prep|Low-copy plasmid prep]]
* [[Griffitts:Gel electrophoresis|Gel electrophoresis]]
* [[Griffitts:Gel electrophoresis|Gel electrophoresis]]
* [[Griffitts:Restriction digest|Restriction digest]]
* [[Griffitts:Restriction digest|Restriction digest]]
* [[Griffitts:In-gel ligation|In-gel ligation]]
* [[Griffitts:In-gel ligation|In-gel ligation]]
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* [[Griffitts:DNA sequencing|Sequencing prep]]
 
==RNA==
==RNA==

Revision as of 16:33, 17 January 2008

Home     Research     Lab Members     Protocols     Publications     Internal    


Contents

Recipes

DNA

PCR

DNA prep

RNA

  • Trizol purification (bacterial)
  • DNAse treatment
  • Reverse transcription
  • 5'RACE

Protein

  • Gel recipes
  • Sample buffer
  • Bacterial lysis
  • 6His purification (6His)
  • MBP purification (MBP)

Bacteria

Plants

Personal tools