Griffitts:Protocols: Difference between revisions
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m (→DNA) |
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==DNA== | ==DNA== | ||
===PCR=== | |||
* [[Griffitts:PCR primers|PCR primers]] | * [[Griffitts:PCR primers|PCR primers]] | ||
* [[Griffitts:TaqPCR|Standard PCR]] | * [[Griffitts:TaqPCR|Standard PCR]] | ||
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* [[Griffitts:Arbitrary PCR|Arbitrary PCR]] | * [[Griffitts:Arbitrary PCR|Arbitrary PCR]] | ||
* [[Griffitts:PCR clean-up|PCR clean-up (Qiagen)]] | * [[Griffitts:PCR clean-up|PCR clean-up (Qiagen)]] | ||
* [[Griffitts:DNA sequencing|Sequencing prep]] | |||
===DNA prep=== | |||
* [[Griffitts:Miniprep|Qiagen Miniprep]] | |||
* [[Griffitts:Low-copy plasmid prep|Low-copy plasmid prep]] | |||
* [[Griffitts:Gel electrophoresis|Gel electrophoresis]] | * [[Griffitts:Gel electrophoresis|Gel electrophoresis]] | ||
* [[Griffitts:Restriction digest|Restriction digest]] | * [[Griffitts:Restriction digest|Restriction digest]] | ||
* [[Griffitts:In-gel ligation|In-gel ligation]] | * [[Griffitts:In-gel ligation|In-gel ligation]] | ||
==RNA== | ==RNA== | ||
Revision as of 13:33, 17 January 2008
Recipes
DNA
PCR
- PCR primers
- Standard PCR
- Pfx PCR
- Overlap-extension PCR
- Arbitrary PCR
- PCR clean-up (Qiagen)
- Sequencing prep
DNA prep
RNA
- Trizol purification (bacterial)
- DNAse treatment
- Reverse transcription
- 5'RACE
Protein
- Gel recipes
- Sample buffer
- Bacterial lysis
- 6His purification (6His)
- MBP purification (MBP)
Bacteria
- Media
- Antibiotics
- Tips on streaking/culturing
- Freezing procedure
- Genomic DNA prep
- RNA prep (Trizol)
- ß-Gal/GUS assay
- Triparental Mating
- Transduction
- Competent Cells
- Electroporation
