Griffitts:Protocols

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m (DNA)
m (Bacteria)
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* [[Griffitts:Triparental Mating|Triparental Mating]]
* [[Griffitts:Triparental Mating|Triparental Mating]]
* [[Griffitts:Transduction|Transduction]]
* [[Griffitts:Transduction|Transduction]]
-
* [[Griffitts:Competent Cells|Competent Cells]]
+
* [[Griffitts:Chemocompetent Cells|Chemocompetent Cells]]
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* [[Griffitts:Electroporation|Electroporation]]
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* [[Griffitts:Electrocompetent Cells|Electrocompetent Cells]]
==Plants==
==Plants==

Revision as of 17:18, 28 January 2008

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Contents

Recipes

DNA

PCR

DNA prep

RNA

  • Trizol purification (bacterial)
  • DNAse treatment
  • Reverse transcription
  • 5'RACE

Protein

  • Gel recipes
  • Sample buffer
  • Bacterial lysis
  • 6His purification (6His)
  • MBP purification (MBP)

Bacteria

Plants

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